Topic outline

  • General

  • Course Syllabus PME

    Subject Description 


    1. This course will describe the specific fields of PME (molecular pharming, bioenergy, biopolymer ..) and  basis of the metabolic pathway of the main classes of secondary metabolites in plants : terpenes, alkaloids, flavonoids and will describe their roles and their pharmacological uses

    2. The different engineering strategies using genes encoding enzymes or regulatory genes will be presented and exemplified.

    3. The main systems used for the production of valuable secondary metabolites will be described: bioconversions, cell suspensions, hairy root cultures...Some examples of industrial processes will be presented, from the plant to the final commercialized product.

    4. Practical classes will present the main techniques used to analyze and identify plant secondary metabolites and their bioactive properties 

    Objectives & Out-come learnings

    After completing this course, students will have:

    - Knowledge of the biosynthesis pathway of the main plant secondary metabolites

    - Initiation to methods of analysis and identification of plant secondary metabolites, perform the bioassay

    - Concepts and strategies for molecular plant secondary metabolite engineering


    COURSE CONTENTS & SCHEDULE

    Class

    Contents

    Hours

    Ref./Resources

    Assignment(s)

    Lect.

    Exr.

    Prc.

    1

    Introduction on plant metabolic engineering (PME)

    - Plan of course and different topics on PME

    1

    Prof. Le Tran Binh

    2

    2.1 Principles of PME

      a) Biosynthesis metabolic pathway

      b) Mechanisms of gene expression regulation

    2

    Prof. Le Tran Binh

    2.2. Genetic transformation

    a) Principles of genetic transformation (gene isolation, vector construction, transformation, analysis of GMCs, )

    b) RNA interference technology ...

    3

    Prof. Le Tran Binh

    2.3. Tissue culture for ME

    (Bioconversion, hairy root culture, cell suspension cultulture

    3

    Prof. Le Tran Binh

    3

    Specific fields of PME (1)

    - Molecular (Protein) farming

    - Biopolymer

    - Bioenergy from plant

    6

    Prof. Le Tran Binh

    4

    4.1. Biosynthesis pathway of some secondary metabolites in plant

    3

    Dr. TTM Huong

    4.2. Case study:  Engineered strategies on manipulating of plant oil composition

    9

    Dr. TTM Huong

    5

    5.1 Methods for identification and analyse plant secondary metabolites and bioactive properties

    5.2 Visite the hairy root laboratory + bioassay laboratory

    3

    Prof. Le Tran Binh

    Total

    27

    0

    3


    Reference Literature:


    [1].   Gregory N. Stephanopoulos et al. Metabolic Engineering: Principles and Methodologies.  1st Edition

    [2]. Christina Smolke. The Metabolic Pathway Engineering Handbook: Tools and Applications

    • Chapter 1: Introduction to PME

      1. Different systems for biomolecules production. What is the advantages when using plants as bioreactor ?

      2. Plant metabolome. Different classes of secondary metabolites in plant 

      3. Why should one like to alter the metabolism of plants


      • Chapter 2: Different tools and strategies to intervene the metabolic pathway

        A: Different approaches to modify the metabolic pathway
        1.Promoter engineer
        2.Transcription factor engineer
        3.GeneOverexpression
        B : Different tools to modify the metabolic pathway
        1.Chromosomal Engineering Strategies
        2.Regulating Gene Expression through Engineered RNA Technologies
        3.Tools Designed to Regulate Translational Efficiency

        • Chapter 3: Illustrated examples

          1.Cyclodextrine produce from tubers
          2.Golden Rice
          3.Production of recombinant Hirudin from seeds

          4.Production of bryodin in tobacco plant

          • Chapter 4: Case studies with plant oils

            1.Increase the DHA/EPA content
            2.Ultra-high erucic acid
            3.Reduce the Ricinoleic acid content in Ricine plant

            4. Biolubricant in lin tree