The goal of the course "Molecular Engineering” is to provide the students with theoretical and practical concepts making possible to obtain a protein (enzyme, binder, etc) with desired characteristics. The first part of the course aims at familiarising the students with the necessary tools: molecular modelling, cloning, expression, purification and characterisation of proteins and mutants. The second part of the course deals with typical examples concerning the design and activity of industrial enzymes, or pharmacological targets with desired properties. In the last part, we will describe some interesting aspects of modular viral proteins. Within the practical course, students will use molecular graphics and modelling softwares and the production of proteins in E. coli, from cloning to purification, and will characterize a mutant with desired activity designed by in-silico approach. Finally, the project will make it possible for students to apply their theoretical and practical skills to a real case.
Acquired Skills :
-Understanding 3D structures and use of molecular modellig softwares
-Cloning, expression, purification and characterisation of proteins and mutants
-Design and production of a mutated protein with desired characteristics.